anti-VEGF-A antibody product blog
Tags: Antibody; VEGF-A; Monoclonal Antibody; anti-VEGF-A antibody;
The VEGF-A vegfa (Catalog #MBS690648) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The Rat Anti-Mouse VEGF-A reacts with Mouse and may cross-react with other species as described in the data sheet. MyBioSource\'s VEGF-A can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC).IHC (paraffin): 1:50-1:100
WB: 1:400-1:8000. Researchers should empirically determine the suitability of the VEGF-A vegfa for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The VEGF-A vegfa product has the following accession number(s) (GI #160358799) (NCBI Accession #NP_001020421.2) (Uniprot Accession #Q00731). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Mouse VEGF is a potent growth and angiogenic cytokine. It stimulates proliferation and survival of endothelial cells, and promotes angiogenesis and vascular permeability. Expressed in vascularized tissues, VEGF plays a prominent role in normal and pathological angiogenesis. Substantial evidence implicates VEGF in the induction of tumor metastasis and intra-ocular neovascular syndromes. VEGF signals through the three receptors; fms-like tyrosine kinase (flt-1), KDR gene product (the murine homolog of KDR is the flk-1 gene product) and the flt4 gene product. Recombinant murine VEGF (isoform 164) is a 39.0 kDa disulfide-linked homodimeric protein consisting of two 164 amino acid polypeptide chains.
Antibody Generation: This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a mouse immunized with mouse VEGF164 recombinant protein). The IgG2 fraction of culture supernatant was purified by Protein G affinity chromatography. Reconstitution: Reconstitute the antibody with 500 ul sterile PBS and the final concentration is 200 ug/ml. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing VEGF-An are readily searchable from our website. Different antibodies against the same target such as VEGF-A may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. Blood, Brain, Embryonic Tissue, Eye, Heart, Kidney, Liver, Lung, Muscle, Skin tissues are correlated with this protein. The following patways have been known to be associated with this gene. Choroidal Neovascularization, Diabetic Retinopathy, Heart Diseases, Inflammation, Macular Degeneration, Macular Edema, Neoplasm Metastasis, Neoplasms, Neovascularization, Pathologic, Retinal Diseases are some of the diseases may be linked to Rat Anti-Mouse VEGF-A.