anti-VIM antibody product blog
Tags: Antibody; Vimentin; VIM; Monoclonal Antibody; anti-VIM antibody;
The VIM vim (Catalog #MBS570247) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse anti Vimentin, conjugated with HRP reacts with Canine, Chicken, Caprine, Hamster, Human, Monkey, Mouse, Rat, Swine, Zebrafish and may cross-react with other species as described in the data sheet. MyBioSource\'s Vimentin can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC), Immunocytochemistry (IHC), Immunohistochemistry (IHC) (frozen), Immunohistochemistry (IHC) (paraffin), Western Blot (WB).RV202 is suitable for immunoblotting, immunocytochemistry, immunohistochemistry on frozen and paraffin-embedded tissues and flow cytometry. Optimal antibody dilution should be determined by titRation; recommended range is 1:100 - 1:200 for flow cytometry, and for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting appliCations. Researchers should empirically determine the suitability of the VIM vim for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The VIM vim product has the following accession number(s) (GI #62414289) (NCBI Accession #NP_003371.2) (Uniprot Accession #P08670). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Mouse anti Vimentin, conjugated with HRP with the following immunoassay(s):
Testing Data (Figure 1: MUB1900P: Immunohistochemistry on frozen section of swine colon showing positive staining in connective tissue cells and no reactivity in epithelial cells )
Testing Data (Figure 2: MUB1900L1: Frozen section of swine colon immunostained with RV202-FITC (1:200) )
Testing Data (Figure 3: MUB1900L2: Frozen section of swine colon immunostained with RV202-HRP (1:200) )
Testing Data (Figure 4: Immunohistochemistry on paraffin section of human colon )
Testing Data (Figure 5: Immunofluorescence staining of the developing neural tube in a 2 days old zebrafish embryo. Left panel: DAPI-staining of cell nuclei, providing an overview of the tissue section used for immunostaining )
Testing Data (Figure 6: Immunofluorescence staining of a 1 month old zebrafish embryo )
Vimentin (57 kDa) is the intermediate filament protein (IFP) of mesenchymal cells. This IFP however often deviates from the tissue-specific and developmentally regulated pattern of expression. Besides its typical expression in most cultured cells, vimentin is also expressed together with several other IFPs during early stages of development. As differentiation proceeds, vimentin is exchanged for the tissue-specific intermediate filament type. Also in cancers, vimentin is often expressed in addition to the tissue-specific IFP.
Conjugation: HRP
Source Note: RV202 is a Mouse monoclonal IgG1 antibody derived by fusion of SP2/0-Ag14 Mouse myeloma cells with spleen cells from a BALB/c Mouse immunized with a cytoskeletal vimentin extract of calf lens.