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anti-WT1 antibody product blog

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Posted on 2018-03-11 07:19:43 by mybiosource_staff
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Tags: Antibody; Wilms Tumor Protein; WT1; Monoclonal Antibody; anti-WT1 antibody;
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The WT1 wt1 (Catalog #MBS179182) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Wilms Tumor Protein Rabbit Monoclonal Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. MyBioSource\'s Wilms Tumor Protein can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Western Blot (WB).
WB: 1:500-1:1000
IHC: 1:50-1:200
ICC/IF: 1:50-1:200
FC/FACS: 1:50. Researchers should empirically determine the suitability of the WT1 wt1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The WT1 wt1 product has the following accession number(s) (GI #65507714) (NCBI Accession #NP_000369.3) (Uniprot Accession #P19544). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Anti-Wilms Tumor Protein Rabbit Monoclonal Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of WT1 expression in K562 cell lysate (MBS179182).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-WT1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for WT1 )
Western Blot (WB) WT1.

Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded mouse kidney, using Wilms Tumor Protein Antibody(MBS179182)
WT1 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-WT1 Antibody (MBS179182)overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
)
Immunohistochemistry (IHC) WT1.

Transcription factor that plays an important role in cellular development and cell survival. Regulates the expression of numerous target genes, including EPO. Plays an essential role for development of the urogenital system. Recognizes and binds to the DNA sequence 5\'-CGCCCCCGC-3\'. It has a tumor suppressor as well as an oncogenic role in tumor formation. Function may be isoform-specific: isoforms lacking the KTS motif may act as transcription factors. Isoforms containing the KTS motif may bind mRNA and play a role in mRNA metabolism or splicing. Isoform 1 has lower affinity for DNA, and can bind RNA.

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