anti-XRCC5 antibody product blog
Tags: Antibody; Monoclonal Antibody; XRCC5; anti-XRCC5 antibody; Ku80;
The Ku80 xrcc5 (Catalog #MBS475194) is an Antibody produced from MouseSource: Human and is intended for research purposes only. The product is available for immediate purchase. The Anti-Ku80 Mouse mAb reacts with Human, Monkey and may cross-react with other species as described in the data sheet. MyBioSource\'s Ku80 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunoprecipitation (IP).
WB: 1:1000
ICC: 1:400
IP: 1:100. Researchers should empirically determine the suitability of the Ku80 xrcc5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The Ku80 xrcc5 product has the following accession number(s) (GI #10863945) (NCBI Accession #NP_066964.1) (Uniprot Accession #P13010). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Anti-Ku80 Mouse mAb with the following immunoassay(s):
Western Blot (WB) (Western blot detection of Ku80 in COS7, Jurkat, Hela, MCF7 and A549 cell lysates using Ku80 mouse mAb (1:1000 diluted). Predicted band size:86KDa. Observed band size:86KDa.)
Immunofluorescence (IF) (Immunofluorescent analysis of Hela cells using Ku80 mouse mAb (1:400).)
Immunoprecipitation (IP) (Immunoprecipitation analysis of Hela cell lysates using Ku80 mouse mAb.)
Entrez Summary: The protein encoded by this gene is the 80-kilodalton subunit of the Ku heterodimer protein which is also known as ATP-dependant DNA helicase II or DNA repair protein XRCC5. Ku is the DNA-binding component of the DNA-dependent protein kinase, and it functions together with the DNA ligase IV-XRCC4 complex in the repair of DNA double-strand break by non-homologous end joining and the completion of V (D)J recombination events. This gene functionally complements Chinese hamster xrs-6, a mutant defective in DNA double-strand break repair and in ability to undergo V (D)J recombination. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity. (provided by RefSeq, Jul 2008).
Immunogen: Purified recombinant human Ku80 protein fragments expressed in E Coli
Sensitivity: This antibody detects endogenous levels of Ku80 and does not cross-react with related proteins.
Sequence Similarities: Belongs to the ku80 family. Contains 1 Ku domain. Subunit Structure: Heterodimer of a 70 kDa and a 80 kDa subunit. The dimer associates in a DNA-dependent manner with PRKDC to form the DNA-dependent protein kinase complex DNA-PK, and with the LIG4-XRCC4 complex. The dimer also associates with NAA15, and this complex displays DNA binding activity towards the osteocalcin FGF response element (OCFRE). In addition, the 80 kDa subunit binds to the osteoblast-specific transcription factors MSX2 and RUNX2. Interacts with ELF3. May interact with APLF. The XRCC5/6 dimer associates in a DNA-dependent manner with APEX1. Identified in a complex with DEAF1 and XRCC6.
Subcellular Location: Nucleus. Nucleus; nucleolus. Chromosome.