|The YDJ1 ydj1 (Catalog #MBS802740) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Hsp40, YDJ1 Antibody: Biotin reacts with Yeast. Does not cross react with Human, Mouse or Rat. and may cross-react with other species as described in the data sheet. MyBioSource\'s Hsp40 (YDJ1) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), ELISA (EIA).
1:2000 (WB). Researchers should empirically determine the suitability of the YDJ1 ydj1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The YDJ1 ydj1 product has the following accession number(s) (GI #6324265) (NCBI Accession #NP_014335.1) (Uniprot Accession #P25491). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Hsp40, YDJ1 Antibody: Biotin with the following immunoassay(s):
Western Blot (WB) (Western Blot analysis of Yeast recombinant cell lysate showing detection of Hsp40 protein using Mouse Anti-Hsp40 Monoclonal Antibody, Clone 2A7.H6. Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp40 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.)
Background Info: Detects ~40kDa.
Scientific Background: Human Hsp40/DnaJ proteins comprise a large protein family, members of which feature the J domain (named after the bacterial DnaJ protein) (1). The J-domain spans the first 75 N-terminal amino acids and is separated from the C-terminal by a glycine/phenylalanine-rich domain (2). Members of the Hsp40/DnaJ family play diverse roles in many cellular processes, such as folding, translocation, degradation and assembly of multi-protein complexes. In particular, Hdj1, the first human Hsp40/DnaJ protein identified, plays an important role in protein translation and folding, as well as in the regulation of Hsp70 function (3). HSP40 stimulates the ATPase activity of HSP70 which in turn causes conformational changes of the unfolded proteins (4, 5). The Hsp40-Hsp70-unfolded protein complex further binds to co-chaperones Hip, Hop and HSP90 which leads to protein folding, or components of protein degradation machinery CHIP and BAG-1 (6). Some studies have shown that the difference between HDJ1 and type 1 DNAJ proteins including HDJ2 and yeast YdjI is the result of the possession of a zinc finger domain by the latter, which helps in the function of protein folding. (7, 8).
Conjugate: Biotin. Storage Buffer: 50% glycerol, 0.09% sodium azide. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing YDJ1 are readily searchable from our website. Different antibodies against the same target such as YDJ1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.