GAD1 antibody product blog
Tags: Antibody; GAD1; GAD1/GAD67; GAD1 antibody;
The GAD1 gad1 (Catalog #MBS4380441) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The GAD1/GAD67 (GABAergic Neuronal Marker) reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s GAD1/GAD67 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC) Formalin.ELISA (For coating, order antibody without BSA)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95�C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined. Researchers should empirically determine the suitability of the GAD1 gad1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The GAD1 gad1 product has the following accession number(s) (GI #58331246) (NCBI Accession #NP_000808.2) (Uniprot Accession #Q99259). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the GAD1/GAD67 (GABAergic Neuronal Marker) with the following immunoassay(s):
Immunohistochemistry (IHC) (Formalin-fixed, paraffin-embedded human Brain stained with GAD1 (GAD67) Mouse Monoclonal Antibody (GAD1/2391).)
SDS-Page (SDS-PAGE Analysis Purified GAD1 (GAD67) Mouse Monoclonal Antibody (GAD1/2391).Confirmation of Integrity and Purity of Antibody.)
Western Blot (WB) (Western Blot Analysis of human Brain tissue lysate using GAD1 (GAD67) Mouse Monoclonal Antibody (GAD1/2391).)
Testing Data (Analysis of Protein Array containing more than 19, 000 full-length human proteins using GAD1 (GAD67) Mouse Monoclonal Antibody (GAD1/2391) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD\'s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD\'s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)
Immunogen: Recombinant human GAD1 (GAD67) protein fragment (around aa 72-135)
Cellular Localization: Cytoplasmic.