HLA-DRB1 antibody product blog
Tags: Antibody; HLA-DRB1; HLA-DRB; HLA-DRB1 antibody;
The HLA-DRB1 hla-drb1 (Catalog #MBS438800) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The HLA-DRB (MHC II) Mouse Monoclonal Antibody reacts with Human, Monkey and may cross-react with other species as described in the data sheet. MyBioSource\'s HLA-DRB can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC) Formalin.Flow Cytometry (0.5-1ug/million cells)
Immunofluorescence (0.1-4ug/ml)
Western Blot (0.5-2ug/ml)
Immunohistochemistry (Formalin-fixed) (0.25-0.5ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined. Researchers should empirically determine the suitability of the HLA-DRB1 hla-drb1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The HLA-DRB1 hla-drb1 product has the following accession number(s) (GI #155030211) (NCBI Accession #NP_002115.2) (Uniprot Accession #P01911). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the HLA-DRB (MHC II) Mouse Monoclonal Antibody with the following immunoassay(s):
Western Blot (WB) (Western Blot Anaysis of Ramos Cell Lyste using HLA-DRB Monoclonal Antibody (HLA-DRB/1067).)
Immunohistochemistry (IHC) (Formalin-fixed, paraffin-embedded human Histiocytoma stained with HLA-DRB Monoclonal Antibody (HLA-DRB/1067).)
Immunohistochemistry (IHC) (Formalin-fixed, paraffin-embedded human Tonsil stained with HLA-DRB Monoclonal Antibody (HLA-DRB/1067).)
Western Blot (WB) (Western Blot Analysis of Ramos cell and human spleen tissue lysate using HLA-DR MAb (HLA-DRB/I067).)
Immunofluorescence (IF) (Immunofluorescence Analysis of Raji cells labeling HLA-OR with HLA-DR Monoclonal Antibody (HLA-DRB/I067) conjugated with APC (Green). The nuclear counterstain is Reddot (Red))
Flow Cytometric (Flow Cytometric Analysis of human Raji cells using HLA-DR MAb (SPM423) followed by Goat anti-mouse I G-CF488 (Blue); Isotype Control (Red).)
Cellular Localization: Cell Surface
Immunogen: Activated human peripheral blood mononuclear cells. Hu-Chromosome Location: 6p21.3
Positive Control: Ramos, Daudi or HuT78 cells. Tonsil or lymph node. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing HLA-DRB1 are readily searchable from our website. Different antibodies against the same target such as HLA-DRB1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.