VIL1 antibody product blog
Tags: Antibody; Villin; VIL1; VIL1 antibody;
The VIL1 vil1 (Catalog #MBS4380278) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Villin (GI-Mucosal & Urogenital Brush Border Marker) reacts with Human. Others not known. and may cross-react with other species as described in the data sheet. MyBioSource\'s Villin can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC) Formalin.FC/FACS: 0.5-1ug/million cells in 0.1ml
IF: 1-2ug/ml
WB: 1-2ug/ml for 60 minutes at RT
IHC-F: 0.25-0.5ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Researchers should empirically determine the suitability of the VIL1 vil1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The VIL1 vil1 product has the following accession number(s) (GI #194394237) (NCBI Accession #NP_009058.2) (Uniprot Accession #P09327). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Villin (GI-Mucosal & Urogenital Brush Border Marker) with the following immunoassay(s):
Immunohistochemistry (IHC) (Formalin-fixed, paraffin-embedded human Rectum stained with Villin Monoclonal Antibody (VIL1/1325).)
Immunohistochemistry (IHC) (Formalin-fixed, paraffin-embedded human Colon stained with Villin Monoclonal Antibody (VIL1/1325).)
Western Blot (WB) (Western Blot of A549, HepG2 & HCT116 Cell Lysates with Villin Monoclonal Antibody (VIL1/1325).)
Immunogen: Recombinant human Villin fragment of 133 amino acid residues (aa179-311)
Cellular Localization: Cytoplasmic & Cell Surface. Hu-chromosome Location: 2q35
Positive Control: A549, HepG2 and HCT116 cells. Colon or Rectum. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing VIL1 are readily searchable from our website. Different antibodies against the same target such as VIL1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. Carcinoma, Hepatocellular, Disease Models, Animal, Inflammation, Liver Diseases, Liver Neoplasms, Lung Neoplasms, Ovarian Neoplasms, Uterine Cervical Neoplasms are some of the diseases may be linked to Villin (GI-Mucosal & Urogenital Brush Border Marker). Brain, Embryonic Tissue, Intestine, Kidney, Liver, Lung, Muscle, Stomach, Uterus tissues are correlated with this protein. The following patways have been known to be associated with this gene.