WT1 antibody product blog
Tags: Antibody; WT1; Wilm's Tumor 1; WT1 antibody;
The WT1 wt1 (Catalog #MBS439717) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Wilm\'s Tumor 1 (WT1) (Wilm\'s Tumor & Mesothelial Marker) Mouse Monoclonal Antibody reacts with Human and Rat. Others-not known and may cross-react with other species as described in the data sheet. MyBioSource\'s Wilm\'s Tumor 1 (WT1) can be used in a range of immunoassay formats including, but not limited to, mmunohistology (Formalin-fixed).Suggested Dilutions: Immunohistology (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined. Researchers should empirically determine the suitability of the WT1 wt1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The WT1 wt1 product has the following accession number(s) (GI #65507714) (NCBI Accession #NP_000369.3) (Uniprot Accession #P19544). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Wilm\'s Tumor 1 (WT1) (Wilm\'s Tumor & Mesothelial Marker) Mouse Monoclonal Antibody with the following immunoassay(s):
Immunohistochemistry (IHC) (Formalin-fixed, paraffin-embedded Human Mesothelioma stained with Wilm�s Tumor Monoclonal Antibody (WT1/857 + 6F-H2))
Immunogen Recombinant: full length human WT1 protein (WT1/857); Recombinant fragment aa1-181 of human WT1 (6F-H2)
Cellular Localization: Nuclear and Cytoplasmic
Positive Control K562 cells. Wilm�s Tumor, mesothelioma or fetal kidney. Supplied As: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 1mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing WT1 are readily searchable from our website. Different antibodies against the same target such as WT1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. Breast Neoplasms, Denys-Drash Syndrome, Disease Models, Animal, Glomerulonephritis, Kidney Diseases, Leukemia, Leukemia, Myeloid, Acute, Lung Neoplasms, Renal Insufficiency, Wilms Tumor are some of the diseases may be linked to Wilm\'s Tumor 1 (WT1) (Wilm\'s Tumor & Mesothelial Marker) Mouse Monoclonal Antibody. WT1 also interacts with the following gene(s): FOXD1, NPHS1, NR0B1, PAWR, PAX2, PAX8, SOX9, TP53, WNT4, WTAP.