ZAP70 antibody product blog
Tags: Antibody; ZAP70; ZAP70 antibody;
The ZAP70 zap70 (Catalog #MBS438932) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The ZAP70 (Chronic Lymphocytic Leukemia Marker) Mouse Monoclonal Antibody reacts with Human.Others not known and may cross-react with other species as described in the data sheet. MyBioSource\'s ZAP70 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC) Formalin.
Flow Cytometry (0.5-1ug/million cells in 0.1ml)
Immunofluorescence (0.5-1ug/ml)
Immunohistochemistry (Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes). Researchers should empirically determine the suitability of the ZAP70 zap70 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The ZAP70 zap70 product has the following accession number(s) (GI #31455611) (NCBI Accession #NP_001070.2) (Uniprot Accession #P43403). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the ZAP70 (Chronic Lymphocytic Leukemia Marker) Mouse Monoclonal Antibody with the following immunoassay(s):
Immunohistochemistry (IHC) (Formalin-fixed, paraffin-embedded human Tonsil stained with ZAP70 Monoclonal Antibody (2F3.2).)
Cellular Localization: Cytoplasmic
Immunogen: Recombinant ZAP-70 protein including residues 1-254 and encompassing SH2 domains of human ZAP70. Hu-Chromosome Location: 2q12
Positive Control: Jurkat cells. Tonsil or lymph node. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing ZAP70 are readily searchable from our website. Different antibodies against the same target such as ZAP70 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. Blood, Bone Marrow, Brain, Intestine, Kidney, Liver, Lymph Node, Prostate, Spleen, Thymus tissues are correlated with this protein. The following patways have been known to be associated with this gene. ZAP70 also interacts with the following gene(s): CBL, CD247, CD3E, LAT, LCK, LCP2, PLCG1, SHC1, VAV1. Abnormalities, Multiple, Cell Transformation, Neoplastic, Immunologic Deficiency Syndromes, Inflammation, Kidney Diseases, Necrosis, Neoplasms, Experimental, Neutropenia, Severe Combined Immunodeficiency, Vascular Diseases are some of the diseases may be linked to ZAP70 (Chronic Lymphocytic Leukemia Marker) Mouse Monoclonal Antibody.