anti-ATPase alpha 3, Na, K antibody product blogThe ATPase alpha 3, Na, K n/a (Catalog #MBS609226) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The ATPase alpha 3, Na, K reacts with Bovine, Canine, Guinea Pig, Human, Mouse, Rabbit, Rat, Sheep and may cross-react with other species as described in the data sheet. MyBioSource\'s ATPase alpha 3, Na, K can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Neutralization.
Suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry and Blocking/Neutralization.
Dilution: Immunofluorescence (IC): 5ug/ml
Western Blot: 1ug/ml detects a band at~110kD from canine skeletal muscle extract.
Immunohistochemistry (Frozen): 3ug/ml, Staining of rat retina yeilds a pattern consistant with plasma membrane localization.
Immunohistochemistry (Paraffin): 1:10-1:2000
Blocking/Neutralization: 1:10. Researchers should empirically determine the suitability of the ATPase alpha 3, Na, K n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
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The sodium/potassium ATPase is an integral membrane enzyme found in all cells of higher organisms and is responsible for the ATP-dependent transport of sodium and potassium across the cell membrane. This membrane-bound enzyme is related to a number of other ATPases including sarcoplasmic and endoplasmic reticulum calcium ATPase (SERCA) and plasma membrane calcium ATPase (PMCA). The sodium/potassium ATPase consists of a large, multipass, transmembrane catalytic subunit, termed the alpha subunit, and an associated smaller glycoprotein, termed the beta subunit. Studies indicate that there are three isoforms of the alpha subunit (alpha 1, alpha 2, alpha 3) and two isoforms of the beta subunit (beta 1 and beta 2) encoded by two multigene families. The different isoforms of the sodium/potassium ATPase exhibit tissue-specific and developmental patterns of expression. The alpha 1 and beta mRNAs are present in all cell types examined, whereas the alpha 2 and alpha 3 mRNAs exhibit a more restricted pattern of cell-specific expression. The alpha-3 subunit has been found in neuronal and to skeletal and cardiac muscle, lung and stomach tissues.
Immunogen: Canine cardiac microsomes. Marker: Plasma membrane. Positive Control: Human tonsil and prostate, rat retina. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing ATPase alpha 3, Na, K are readily searchable from our website. Different antibodies against the same target such as ATPase alpha 3, Na, K may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.