anti-CD52 antibody product blogThe CD52 n/a (Catalog #MBS219952) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s CD52 can be used in a range of immunoassay formats including, but not limited to, Flow cytometry (FC/FACS), Immunohistology Paraffin.
Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul
Flow Cytometry: Minimum Dilution: 1/25; Maximum Dilution: 1/200. Researchers should empirically determine the suitability of the CD52 n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
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Mouse anti Pig CD52, clone 11/305/44 is a monoclonal antibody recognizing the porcine homologue of human CD52, a 19 kDa antigen expressed by mature lymphocytes, monocytes and dendritic cells.Clone 11/305/44 was originally clustered at the 1st International Swine Cluster of Differentiation Workshop held in 1992 as SWC1 (Lunney et al.1994). Further studies have demonstrated that SWC1 is the porcine orthologue CD52, which is expressed by most leucocytes including resting T-cells, monocytes and granulocytes, but is not expressed by B-cells, erythrocytes or platelets (Piriou-Guyzlack et al. 2008) & (Leitner et al. 2012). Porcine CD52, expressed at very much higher levels on monocytes than mature macrophages, and SWC9, expressed exclusively on mature tissue macrophages, have been used as markers of monocyte-macrophage differentiation (Sanchez et al. 1999) & (McCollough et al. 1999).
Perservative Stabilisers: 0.09% Sodium Azide (NaN3)
Preparation: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant. Buffer Solution: Phosphate buffered saline
Target Species: Pig. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing CD52 are readily searchable from our website. Different antibodies against the same target such as CD52 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.