anti-CEACAM1 antibody product blogThe CEACAM1 ceacam1 (Catalog #MBS468039) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The anti-human CEACAM1 monoclonal antibody reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s CEACAM1 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), CELISA and Immunohistology on cryosections.
Immunofluorescence: genetic immunisation with cDNA encoding the extracellular region of human CEACAM1-A2
Immunohistology: 10 ug/ml (on cryosections) For each application a titration should be performed to determine the optimal concentration.
ELISA: 1:200 - 1:400
Flow Cytometry: 1.2 ug/10^6 cells
CELISA: 1:200 - 1:400. Researchers should empirically determine the suitability of the CEACAM1 ceacam1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The CEACAM1 ceacam1 product has the following accession number(s) (GI #68161541) (NCBI Accession #NP_001020083.1) (Uniprot Accession #P13688). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
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Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the anti-human CEACAM1 monoclonal antibody with the following immunoassay(s):
Testing Data (Fig.1: FACS analysis of BOSC23 cells using GM-8G5 Cat.# GM-0501. BOSC23 cells were transiently transfected with an expression vector encoding either CEACAM1 (red curve) or an irrelevant protein (control transfectant: black curve). Binding of GM-8G5 was detected with a PE-conjugated secondary antibody. A positive signal was obtained only with CEACAM1 transfected cells.)
Testing Data #2 (Fig.2: Immunohistochemical staining on cryosections of colon tissue using GM-8G5 Cat.# GM-0501. PBS containing 2.5% horse serum served as negative control (A). Detection of GM-8G5 occurred with a biotinylated anti-mouse-IgG secondary antibody and a streptavidin-peroxidase conjugate (B). Diaminobenzidine was used as substrate. Nuclei were stained with hematoxilin.)
Testing Data #3 (Fig3: Members of the CEA family were expressed on BOSC cells after transient transfection with expression vectors containing either the cDNA of CEACAM1,5,6,7,8,19,20 or 21. Recognition of CEACAM3 and 4 was tested on stably transfected HeLa (CEACAM3) and CHO cells (CEACAM4). Expression of the constructs was confirmed with monoclonal antibodies known to recognise the corresponding proteins (CEACAM1,3,4: D14HD11; CEACAM5: 26/3/13; CEACAM6: 9A6; CEACAM7: BAC2; CEACAM8: GM-2H6; CEACAM19-21: anti-myc; green curves). An irrelevant monoclonal antibody served as a negative control (black curves). For specificity testing, protein G-purified GM-8G5 was tested on all CEACAM transfectants. A positive signal was only obtained with CEACAM1-expressing cells (red curves).)
Background: CEACAM1 (BGP/CD66a) is a transmembrane glycoprotein which belongs to the carcinoembryonic antigen (CEA) gene family (1,2). It is expressed on cells of epithelial and myeloid origin and mediates homophilic intercellular interactions that influence cellular growth, immune cell activation, and tissue morphogenesis. CEACAM1 is a putative tumour suppressor based on diminished expression in some aggressive types of cancer cells (3). The anti-tumour effect may be due to inhibition of tumour angiogenesis, possibly by increased secretion of anti-angiogenic molecules from the cells (4). Like all members of the CEACAM family, it consists of a single N domain, with structural homology to the immunoglobulin variable domains, followed by two immunoglobulin constant-like A (A1, A2) and one B domain. While the N, A1 and B domains can also be found in other CEA-family members, the A2 domain of CEACAM1 differs from those found in other CEACAM.