anti-GFP antibody product blogThe GFP n/a (Catalog #MBS475753) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s GFP can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). Researchers should empirically determine the suitability of the GFP n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Anti-GFP Mouse mAb with the following immunoassay(s):
Western Blot (WB) (Western blot analysis using GFP mouse mAb against recombinant GFP fusion protein (1) and various recombinant fusion protein with GFP tag (2, 3, 4).)
Western Blot (WB) (Western blot analysis using GFP mouse mAb against extracts from HCC827 cells, untransfected (1) and transfected with GFP (2).)
Flow Cytometry (FC/FACS) (Flow cytometric analysis of HCC827 cells, untransfected (blue) or transfected with GFP (green), using GFP mouse mAb.)
Immunocytochemistry (ICC) (ICC analysis of HCC827 cells, untransfected (left) or transfected with GFP (right) using GFP mouse mAb.)
Entrez Summary: GFP (Green fluorescence protein) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light when excited by blue light. GFP cDNA produces a fluorescent product when expressed in prokaryotic cells, without the need for exogenous substrates or cofactors. GFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. Other applications of GFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. GFP technnology has considerably contributed to a greater understanding of cellular physiology.
Immunogen: Purified recombinant fragment of GFP expressed in E Coli. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing GFP are readily searchable from our website. Different antibodies against the same target such as GFP may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.