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anti-GPI antibody product blog

Tags: Antibody; Monoclonal Antibody; Glucose-6-Phosphate Isomerase; GPI; anti-GPI antibody;

     The GPI gpi (Catalog #MBS9602380) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Glucose-6-phosphate isomerase Antibody reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s Glucose-6-phosphate isomerase can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemisty (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA).
ELISA: 1:10000
WB: 1:500-1:2000
IHC: 1:200-1:1000
ICC: 1:200-1:1000. Researchers should empirically determine the suitability of the GPI gpi for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The GPI gpi product has the following accession number(s) (GI #18201905) (NCBI Accession #NP_000166.2) (Uniprot Accession #P06744). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Glucose-6-phosphate isomerase Antibody with the following immunoassay(s):
Western Blot (WB) (Figure 1: Western blot analysis using GPI mouse mAb against HepG2 (1), SMMC-7721 (2) cell lysate and rat liver tissues lysate (3).)
Western Blot (WB) GPI.

Description: Glucose-6-phosphate isomerase, or phosphoglucose isomerase, also known as GPI. It belongs to the GPI family whose members encode multifunctional phosphoglucose isomerase proteins involved in energy pathways and it is an enzyme that catalyzes the conversion of glucose-6-phosphate into fructose 6-phosphate in the second step of glycolysis. The protein functions in different capacities inside and outside the cell.
Function: Besides it\'s role as a glycolytic enzyme, mammalian GPI can function as a tumor-secreted cytokine and an angiogenic factor (AMF) that stimulates endothelial cell motility. GPI is also a neurotrophic factor (Neuroleukin) for spinal and sensory neurons.
Subunit Structure: Homodimer in the catalytically active form, monomer in the secreted form.
Post-translational Modifications: Phosphorylation at Ser-185 by CK2 has been shown to decrease enzymatic activity and may contribute to secretion by a non-classical secretory pathway. ISGylated.
Similarity: Belongs to the GPI family.

Immunogen: Purified recombinant fragment of human Glucose-6-phosphate isomerase expressed in E. Coli
Subcellular Location: Cytoplasm. Secreted. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing GPI are readily searchable from our website. Different antibodies against the same target such as GPI may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.
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