anti-HSPA8 antibody product blogThe HSPA8 hspa8 (Catalog #MBS800271) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Hsc70 (Hsp73) Antibody: Alkaline Phosphatase reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s HSC70 (HSP73) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP), Immunohistochemistry (IHC), PLA.
1:1000 (WB). Researchers should empirically determine the suitability of the HSPA8 hspa8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The HSPA8 hspa8 product has the following accession number(s) (GI #5729877) (NCBI Accession #NP_006588.1) (Uniprot Accession #P11142). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
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Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Hsc70 (Hsp73) Antibody: Alkaline Phosphatase with the following immunoassay(s):
Western Blot (WB) (Western Blot analysis of Human Cell lysates showing detection of Hsc70 protein using Mouse Anti-Hsc70 Monoclonal Antibody, Clone 1F2-H5. Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsc70 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT. 1: mix of 10 different human cell lines, 2: Hsp72 recombinant protein, and 3: Hsc70(Hsp73) recombinant protein.)
Immunocytochemistry/Immunofluorescence (ICC/IF) (Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Hsc70 Monoclonal Antibody, Clone 1F2-H5. Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20 degree C. Primary Antibody: Mouse Anti-Hsc70 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Bright cytoplasmic staining, duller nuclear staining.)
Background Info: Detects ~73kDa. Does not cross react with Hsp70
Scientific Background: Hsp70 genes encode abundant heat-inducible 70-kDa hsps (hsp70s). In most eukaryotes hsp70 genes exist as part of a multigene family. They are found in most cellular compartments of eukaryotes including nuclei, mitochondria, chloroplasts, the endoplasmic reticulum and the cytosol, as well as in bacteria. The genes show a high degree of conservation, having at least 5O% identity (2). The N-terminal two thirds of hsp70s are more conserved than the C-terminal third. Hsp70 binds ATP with high affinity and possesses a weak ATPase activity which can be stimulated by binding to unfolded proteins and synthetic peptides (3). When hsc70 (constitutively expressed) present in mammalian cells was truncated, ATP binding activity was found to reside in an N-terminal fragment of 44 kDa which lacked peptide binding capacity. Polypeptide binding ability therefore resided within the C-terminal half (4). The structure of this ATP binding domain displays multiple features of nucleotide binding proteins (5). When cells are subjected to metabolic stress (e.g., heat shock) a member of the hsp 70 family, hsp 70 (hsp72), is expressed; hsp 70 is highly related to hsc70 (>90% sequence identity). Constitutively expressed hsc70 rapidly forms a stable complex with the highly inducible hsp70 in cells following heat shock. The interaction of hsc70 with hsp 70 is regulated by ATP. These two heat shock proteins move together in the cell experiencing stress. Furthermore, research on hsc70 has implicates it with a role in facilitating the recovery of centrosomal structure and function after heat shock (6).