anti-LAYN antibody product blogThe LAYN layn (Catalog #MBS200287) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The LAYN antibody reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s LAYN can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB).
Recommended starting dilution is 1:500. Researchers should empirically determine the suitability of the LAYN layn for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The LAYN layn product has the following accession number(s) (GI #385719187) (NCBI Accession #NP_001245319) (Uniprot Accession #Q6UX15). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the LAYN antibody with the following immunoassay(s):
Western Blot (WB) (The Recombinant Human LAYN (25ng) and Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human LAYN antibody (1:3000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Recombinant Human LAYN Lane 2.: A549 cell lysate Lane 3.: 293T cell lysate)
Layilin is a widely expressed integral membrane hyaluronan receptor and a transmembrane protein with homology to C-type lectins. This protein interacts with the ERM protein family of cytoskeletal membrane linker molecule members, including talin, ezrin, moesin, and radixin. The carboxy-terminal domain of layilin is capable of binding to the amino-terminal domain of radixin. An interdomain interaction between the amino- and the carboxy-terminal domains of radixin inhibits its ability to bind to layilin. Layilin plays an important role in cell adhesion and motility. It is important for the maintenance of the cortical structure in the cell because it mediates signals from the extracellular matrix to the cytoskeleton. In spreading cells, Layilin acts as a membrane-binding site for talin in the peripheral ruffles, and it may play a role in the migration of cells to a wound site.
Antigen Species: Human
Immunogen: Recombinant human LAYN (22-235aa) purified from E. coli. Clone Note: Anti-human LAYN mAb, clone AT20G8, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human LAYN protein. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing LAYN are readily searchable from our website. Different antibodies against the same target such as LAYN may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.