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anti-Mapk8ip2 antibody product blog

Tags: Antibody; Monoclonal Antibody; anti-MAPK8IP2 antibody; MAPK8IP2; JIP2/IB2;

     The Mapk8ip2 mapk8ip2 (Catalog #MBS801173) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The JIP2/IB2 Antibody: ATTO 633 reacts with Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s JIP2/IB2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC).
1:1000 WB. Researchers should empirically determine the suitability of the Mapk8ip2 mapk8ip2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The Mapk8ip2 mapk8ip2 product has the following accession number(s) (GI #257096008) (NCBI Accession #NP_068740.3) (Uniprot Accession #Q9ERE9). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the JIP2/IB2 Antibody: ATTO 633 with the following immunoassay(s):
Western Blot (WB) (Western Blot analysis of Monkey COS cells transiently transfected with Flagtagged JIP-1 showing detection of ~100 kDa JIP2/IB2 protein using Mouse Anti-JIP2/IB2 Monoclonal Antibody, Clone S135-37. Lane 1: Molecular Weight Ladder. Lane 2: Monkey COS cells transiently transfected with Flagtagged JIP-1. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-JIP2/IB2 Monoclonal Antibody at 1:200 for 16 hours at 4 degree C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~100 kDa.)
Western Blot (WB) Mapk8ip2.

Background Info: Detects a 100 kDa and larger protein. Does not cross-react with JIP-1/IB-1.

Scientific Background: The JNK-interacting protein (JIP) group of scaffold proteins selectively mediates JNK signaling by aggregating specific components of the MAPK cascade to form a functional JNK signaling module. JIP2 inhibits IL1 beta-induced apoptosis in insulin-secreting cells. May function as a regulator of vesicle transport, through interactions with the JNK-signaling components and motor proteins. It is expressed mainly in the cerebellum, pituitary gland, occipital lobe, and the amygdala of the brain, but also in the pancreas, including insulin-secreting cells.

Conjugate: ATTO 633. Storage Buffer: PBS pH 7.4, 50% glycerol, 0.1% sodium azide. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing Mapk8ip2 are readily searchable from our website. Different antibodies against the same target such as Mapk8ip2 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.
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