anti-Syt12 antibody product blogThe Syt12 syt12 (Catalog #MBS801289) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Synaptotagmin-12 Antibody: ATTO 565 reacts with Rat, Mouse and may cross-react with other species as described in the data sheet. MyBioSource\'s Synaptotagmin-12 can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Western Blot (WB), Immunohistochemistry (IHC).
1:1000 (WB). Researchers should empirically determine the suitability of the Syt12 syt12 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The Syt12 syt12 product has the following accession number(s) (GI #19527400) (NCBI Accession #NP_598925.1) (Uniprot Accession #Q920N7). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
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Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Synaptotagmin-12 Antibody: ATTO 565 with the following immunoassay(s):
Western Blot (WB) (Western Blot analysis of Monkey COS cells transfected with GFP-tagged Synaptotagmin showing detection of ~45 kDa Synaptotagmin-12 protein using Mouse Anti-Synaptotagmin-12 Monoclonal Antibody, Clone S277-7. Lane 1: Molecular Weight Ladder. Lane 2: Monkey COS cells transfected with GFP-tagged Synaptotagmin. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Synaptotagmin-12 Monoclonal Antibody at 1:200 for 16 hours at 4 degree C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~45 kDa.)
Background Info: Detects ~45kDa. Does not cross-react with other Synaptotagmin-6 or other Synaptotagmins.
Scientific Background: Synaptotagmins constitute a family of membrane-trafficking proteins that are characterized by an N-terminal transmembrane region (TMR), a variable linker, and two C-terminal C2 domains - C2A and C2B. There are 15 members in the mammalian synaptotagmin family. There are several C2-domain containing protein families that are related to synaptotagmins, including transmembrane (Ferlins, E-Syts, and MCTPs) and soluble (RIMs, Munc13s, synaptotagmin-related proteins and B/K) proteins. The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. Calcium binding to synaptotagmin participates in triggering neurotransmitter release at the synapse. The first domain mediates Ca(2+)-dependent phospholipid binding. The second C2 domain mediates interaction with Stonin 2. Synaptotagmin may have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse. It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone. A Ca(2+)-dependent interaction between synaptotagmin and putative receptors for activated protein kinase C has also been reported. It can bind to at least three additional proteins in a Ca(2+)-independent manner; these are neurexins, syntaxin and AP2 (1, 2).