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anti-TEM7 antibody product blog

Tags: Antibody; Monoclonal Antibody; TEM7; anti-TEM7 antibody;

     The TEM7 plxdc1 (Catalog #MBS245312) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Monoclonal [clone 197C193] (IgG1) to Human TEM7 reacts with Gibbon, Gorilla, Human
Predicted Reactivity: Monkey, Dog (at least 90% immunogen sequence identity) and may cross-react with other species as described in the data sheet. MyBioSource\'s TEM7 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC - Paraffin), Western Blot (WB).
IHC-P (2.5 - 5 ug/ml), WB (1 - 2 ug/ml)
Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti.. Researchers should empirically determine the suitability of the TEM7 plxdc1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The TEM7 plxdc1 product has the following accession number(s) (GI #15011862) (NCBI Accession #NP_065138.2) (Uniprot Accession #Q8IUK5). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Mouse Monoclonal [clone 197C193] (IgG1) to Human TEM7 with the following immunoassay(s):
Immunohistochemistry (IHC) (Anti-TEM7 antibody IHC of human breast, vessels. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
Immunohistochemistry (IHC) TEM7.

Western Blot (WB) (Western blot detection of TEM7 in HCT-116 cell lysate using antibody at 1 µg/ml dilution.)
Western Blot (WB) TEM7.

TEM7 (tumor endothelial marker 7) was identified because it displayed elevated expression during tumor angiogenesis. It is a type 1 membrane protein containing a plexin-like domain.

Target Species: Human
Immunogen Description: Amino acids 409-425 (LQNNLSPKTKGTPVHLG) of human TEM7 were used to develop this monoclonal antibody. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%); Marmoset, Panda, Dog (94%); Horse (88%); Hamster, Elephant, Bovine (82%). Immunogen: TEM7 antibody was raised against amino acids 409-425 (LQNNLSPKTKGTPVHLG) of human TEM7 were used to develop this monoclonal antibody. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%); Marmoset, Panda, Dog (94%); Horse (88%); Hamster, Elephant, Bovine (82%).
Antigen Modification: aa411-427. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing TEM7 are readily searchable from our website. Different antibodies against the same target such as TEM7 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.
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