anti-TPH1 antibody product blogThe TPH1 tph1 (Catalog #MBS175006) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-Tryptophan Hydroxylase antibody (monoclonal) reacts with Human, Rat, Rabbit and may cross-react with other species as described in the data sheet. MyBioSource\'s Tryptophan Hydroxylase can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the TPH1 tph1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The TPH1 tph1 product has the following accession number(s) (GI #116242823) (NCBI Accession #P17752.4) (Uniprot Accession #P17752). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Anti-Tryptophan Hydroxylase antibody (monoclonal) with the following immunoassay(s):
Western Blot (WB) (Anti-Tryptophan Hydroxylase antibody (monoclonal), MBS175006, Western blotting
Lane 1: Rat Brain Tissue Lysate
Lane 2: Rat Brain Tissue Lysate
Immunohistochemistry (IHC) (Applications: Immunohistochemistry
Sample: Wistar rat brain
"The goal was to identify the dorsal raphe in rat brains, an area rich in serotonergic neurons, so that I could determine double-label in that region. I had been using an AB from Abcam, but they stopped making it. Found this product with a google search, and the vendor had a fair return policy. Strong signal that identifies dorsal raphe; aqueductus alone is not sufficient landmark. Great for free floating sections fixed in 4% paraformaldehyde. Probably can be used at less than 1:500.")
Description: Mouse IgG monoclonal antibody for Tryptophan Hydroxylase, tryptophan hydroxylase 1, tryptophan hydroxylase 2 (TPH1, TPH2) detection. Tested with WB in Human, rat, rabbit. No cross reactivity with other proteins. Uniprot ID: P17752
Background: Tryptophan hydroxylase is the rate-limiting enzyme in the synthesis of serotonin(5-hydroxytryptamine, or 5HT). Tryptophan hydroxylase\atalyzes the biopterin-dependent monooxygenation of tryptophan to5-hydroxytryptophan(5HT), which is subsequently decarboxylated to form the neurotransmitter serotonin. Human tryptophan hydroxylase(TPH) is mapped to chromosome 11p15.3-p14 by in situ hybridization.
Immunogen: Recombinant rabbit tryptophan hydroxylase. Contents: Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
Reconstitution: 1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100ug/ml. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing TPH1 are readily searchable from our website. Different antibodies against the same target such as TPH1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.